SpliceBio

splice.bio ↗ ·Barcelona ·Series B

SpliceBio is a clinical-stage genetic medicines company based in Barcelona that uses a proprietary Protein Splicing platform built on engineered inteins from Tom Muir's lab at Princeton University, allowing large genes to be split across dual AAV vectors and reassembled inside cells — overcoming the size limit that blocks conventional gene therapy. Its lead program, SB-007, targets Stargardt disease and is in the Phase 1/2 ASTRA clinical study, alongside a pipeline in ophthalmology, CNS and other genetic diseases, plus a collaboration with Spark Therapeutics worth up to ~$216M. In June 2025 it closed a $135M (€118M) Series B led by EQT Life Sciences and Sanofi Ventures — the largest biotech financing round ever raised in Spain — bringing total funding to over $200M.

Founded
2014
Founders
2
Total funds raised
€174M+
Rounds
3
Investors
10
Last round
Jun 2025
SectorsBiotech, Gene Therapy, Genetic Medicines, Therapeutics, Life Sciences Start-up MapHealthtech & Bio › Drug discovery & therapeutics developers

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Founders

Miquel Vila-Perelló
Co-Founder & Chief Executive Officer
Sílvia Frutos
Co-Founder & Chief Technology Officer

Global models 5

Kriya Therapeutics
United States · www.kriyatx.com ↗
Private AAV gene therapy platform company with an ophthalmology-heavy pipeline, developing its own genetic medicines through the clinic.
$900MComparable
Beacon Therapeutics
United Kingdom · beacontx.com ↗
Clinical-stage AAV gene therapy company focused on inherited retinal diseases, running its own clinical programmes.
$290MComparable
Atsena Therapeutics
United States · atsenatx.com ↗
Clinical-stage ocular gene therapy company that has worked on dual-AAV and capsid approaches for inherited retinal diseases.
$215MComparable
Generation Bio
United States · generationbio.com ↗
Genetic medicines platform company built to get around AAV's payload limits for large genes; this is what it raised in venture money before its IPO.
$225MComparable
4D Molecular Therapeutics
AAV gene therapy platform company with a lead ophthalmology programme, funded by venture money and pharma partnerships before its IPO.
$200MComparable

In the same niche 67 others

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Notes: Most funded first

Investors 10

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Ysios Capital
ysioscapital.com
3 rounds, led 2 · 2020–25 Spain
EQT
eqtgroup.com
Led Series B 2025 Sweden
Asabys Partners
asabys.com
3 rounds · 2020–25 Spain
Criteria Capital Risc
www.caixacapitalrisc.es
Seed 2020 Spain
UCB Ventures
2 rounds, led 1 · 2022–25 Belgium
Sanofi Ventures
Led Series B 2025 United States
Gilde Healthcare
2 rounds · 2022–25 Netherlands
New Enterprise Associates
2 rounds · 2022–25 United States
Novartis Venture Fund
2 rounds · 2022–25 Switzerland
Series B 2025 Switzerland

Funding rounds 3

DateRoundAmountLead

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